Frizzled-6 Antibody

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Frizzled‑6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Frizzled-6 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled-6
His19-Val153
Accession # O60353
Specificity
Detects human Frizzled-6 in direct ELISAs and Western blots.  In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed, and less than 5% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human (rh) Frizzled-5, rmFrizzled-7, rmFrizzled-8, and rhFrizzled-10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF3149 in the following applications:

Publications
Read Publication using
AF3149 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-6 Antibody

  • frizzled (Drosophila) homolog 6
  • frizzled homolog 6 (Drosophila)
  • Frizzled6
  • Frizzled-6
  • FZ6
  • fz-6
  • FZD6
  • Hfz6
  • seven transmembrane helix receptor

Background

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-6 Antibody (AF3149)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Human
(1)
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Review for Frizzled-6 Antibody (AF3149) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3149:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 01/07/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24274766
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24274766

Details

Detection NotesSee PMID 24274766

Comments

CommentsIt works very wellPublished: See PMID 24274766Independent review from antibodyresource.com

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Recent Reviews

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1
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Anonymous
01/07/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol FZD6
Uniprot