Frizzled-5 Overexpression Lysate (Native)

Images

 
Western Blot: Frizzled 5 Overexpression Lysate (Adult Normal) [NBP2-04250] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Frizzled 5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Frizzled-5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_003459. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Frizzled 5, mRNA.
Gene
FZD5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
61.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Frizzled-5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Frizzled-5 Overexpression Lysate (Native)

  • C2orf31
  • chromosome 2 open reading frame 31
  • DKFZP434E2135
  • frizzled (Drosophila) homolog 5
  • frizzled homolog 5 (Drosophila)
  • Frizzled5
  • Frizzled-5
  • Frz-5
  • FZ-5
  • FZD5
  • fzE5
  • HFZ5
  • MGC129692
  • seven-transmembrane receptor frizzled-5
  • Wnt receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
645-WN/CF
Species: Hu, Mu
AF198
Species: Mu
Applications: WB, IHC
AF1505
Species: Hu
Applications: WB, Flow, CyTOF-ready
NLS5103
Species: Hu, Bv, Ca
Applications: IHC-P, ICC
AF1120
Species: Mu
Applications: WB, IHC
NB100-1886
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
MAB194
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
NBP2-33842
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF1620
Species: Mu
Applications: WB, IHC
NBP2-23659
Species: Hu, Mu, Rt, Pm
Applications: WB
MAB1001
Species: Hu, Mu
Applications: Flow, IHC, CyTOF-ready
MAB4605
Species: Hu
Applications: WB, Simple Western, ICC, ICFlow
NBP2-46367
Species: Hu
Applications: WB, IHC
AF3287
Species: Hu, Mu, Rt
Applications: WB
NB100-2439
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for Frizzled-5 Lysate (NBP2-04250) (0)

There are no publications for Frizzled-5 Lysate (NBP2-04250).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Frizzled-5 Lysate (NBP2-04250) (0)

There are no reviews for Frizzled-5 Lysate (NBP2-04250). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Frizzled-5 Lysate (NBP2-04250) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Frizzled-5 Products

Bioinformatics Tool for Frizzled-5 Lysate (NBP2-04250)

Discover related pathways, diseases and genes to Frizzled-5 Lysate (NBP2-04250). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Frizzled-5 Lysate (NBP2-04250)

Discover more about diseases related to Frizzled-5 Lysate (NBP2-04250).
 

Pathways for Frizzled-5 Lysate (NBP2-04250)

View related products by pathway.

PTMs for Frizzled-5 Lysate (NBP2-04250)

Learn more about PTMs related to Frizzled-5 Lysate (NBP2-04250).
 

Research Areas for Frizzled-5 Lysate (NBP2-04250)

Find related products by research area.

Blogs on Frizzled-5

There are no specific blogs for Frizzled-5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Wnt-5a
645-WN-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Frizzled-5 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol FZD5
Entrez