FOXO3 [p Ser253] Antibody (ST49-01)

Western Blot: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Western blot analysis of FOXO3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.

Western Blot: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Analysis of Phospho-FOXO3a(S253) on different lysates using anti-Phospho-FOXO3a(S253) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC12

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: FOXO3 [p Ser253] Antibody (ST49-01) [NBP2-67521] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.

Product Discontinued

FOXO3 [p Ser253] Antibody (ST49-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus aa 238-282 / 673. (SwissProt: O43524 Human; SwissProt: Q9WVH4 Mouse; SwissProt: D3ZBQ1 Rat)
• Modification: p Ser253
• Localization: Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FOXO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FOXO3 [p Ser253] Antibody (ST49-01)

• AF6q21 protein
• AF6q21
• DKFZp781A0677
• FKHRL1
• FKHRL1MGC31925
• FKHRL1P2
• forkhead box O3
• forkhead box protein O3
• forkhead homolog (rhabdomyosarcoma) like 1
• Forkhead in rhabdomyosarcoma-like 1
• forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• FOXO2
• FoxO3
• FOXO3A
• FOXO3Aforkhead box O3A
• MGC12739

Background

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOXO3 Antibody (NBP2-67521)(2)

Publications using NBP2-67521

• Wang W, Yu R, Wu C et al. Berberine alleviates contrast-induced nephropathy by activating Akt/Foxo3a/Nrf2 signalling pathway Journal of cellular and molecular medicine 2023-11-01 [PMID: 37909687] (WB, Rat)
• Yang KJ, Kim JH, Chang YK et al. Inhibition of xanthine oxidoreductase protects against contrast-induced renal tubular injury by activating adenosine monophosphate-activated protein kinase Free Radic. Biol. Med. 2019-09-24 [PMID: 31560952] (WB, Mouse)

Bioinformatics

• Gene Symbol: FOXO3