Follistatin-like 4/FSTL4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Follistatin-like 4/FSTL4 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Follistatin-like 4/FSTL4
  Trp23-Val842
  Accession # NP_055897
• Specificity: Detects human Follistatin-like 4/FSTL4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse FSTL4 is observed and less than 1% cross-reactivity with recombinant human (rh) FSTL1 and rhFSTL5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FSTL4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 4/FSTL4 Antibody [Biotin]

• Follistatin like 4
• Follistatin-like 4
• Follistatin-like protein 4
• follistatin-related protein 4
• FSL4
• FSTL4
• KIAA1061

Background

Follistatin-like 4 (FSTL4) is a presumably secreted member of the follistatin domain-containing protein family (1). Follistatin itself is a potent inhibitor of the TGF-beta superfamily member, activin (1, 2), and this activity is reflected in several other proteins of this family that contain varying numbers of the cysteine-rich follistatin domain. Many of these are matrix proteins such as SPARC and testican for which TGF-beta superfamily inhibition status is presumed but unknown (2, 3). R&D Systems recombinant human FSTL4 inhibits the ability of a TGF-beta superfamily member, BMP-6, to induce osteoblast differentiation of a mouse fibroblast cell line. Human FSTL4 cDNA encodes an 842 amino acid (aa) protein containing a 22 aa signal sequence, a follistatin (EGF- and kazal-like) domain, an EF-hand calcium-binding motif, and two Ig-like cell adhesion molecule-type (IgCAM) domains (1, 4). The second IgCAM domain contains a consensus sequence for FGF/FGF receptor interaction (4). Mature human FSTL4 shares 84%, 82% and 82% aa identity with mouse, rat and bovine FSTL4, respectively. Identity with other follistatin-like family members is mainly within consensus residues of the follistatin domain. Of two reported human FSTL4 isoforms, one is missing the follistatin and EF-hand domains, while the other contains all recognized domains, but is truncated after aa 600 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FSTL4
• Uniprot:
  • NP_055897(Human)