FNBP1 Antibody

Product Discontinued

FNBP1 Antibody Summary

• Immunogen: Peptide with sequence KQLESSKRRFERDC corresponding to internal region according to NP_055848.1.
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FNBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.25 - 2 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human testis lysates (calculated MW of 71.3 kDa band according to NP_055848.1).
  IHC: Paraffin embedded Human Tonsil and Small Intestine.
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FNBP1 Antibody

• FBP17KIAA0554Formin-binding protein 17
• formin binding protein 1
• formin-binding protein 1
• hFBP17
• MGC126804

Background

The protein recognised by this antibody is a member of the formin binding protein family. The protein contains an N terminal Fer/Cdc42 interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled coil domain, a proline rich motif, a second coiled coil domain, a Rho family protein binding domain (RBD), and a C terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FNBP1 Antibody (NB100-1457)(2)

Publications using NB100-1457

• Hu J, Mukhopadhyay A, Craig AW. Transducer of Cdc42-dependent actin assembly promotes epidermal growth factor-induced cell motility and invasiveness. J Biol Chem. 2011-01-21 [PMID: 21062739] (WB)
  
  Details:
  Product cited: FBP17 (IMG-3371). WB: A431 cells transfected with Toca-1 shRNA, Fig 3A. Note: The FBP17 antibody was detected by WB at approximately 72 kDa in A431 cell line, Fig 3A.
• Kamioka Y, Fukuhara S, Sawa H et al. A novel dynamin-associatingFNB molecule, formin-binding protein 17, induces tubular membrane invaginations and participates in endocytosis. J Biol Chem 2004-09-17 [PMID: 15252009]

Bioinformatics

• Gene Symbol: FNBP1
• Entrez:
  • 23048(Human)
• Uniprot:
  • Q96RU3(Human)