FKRP Overexpression Lysate

Images

 
Western Blot: FKRP Overexpression Lysate (Adult Normal) [NBL1-10743] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKRP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FKRP Overexpression Lysate Summary

Description
Transient overexpression lysate of fukutin related protein (FKRP), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200941 and based on accession number NM_024301. The protein contains a C-MYC/DDK Tag.
Gene
FKRP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FKRP Overexpression Lysate

  • EC 2.-
  • fukutin related protein
  • fukutin-related protein
  • LGMD2IFLJ12576
  • MDC1CMGC2991
  • MDDGA5
  • MDDGB5
  • MDDGC5

Background

This gene encodes a protein which is targeted to the medial Golgi apparatus and is necessary for posttranslational modification of dystroglycan. Mutations in this gene have been associated with congenital muscular dystrophy, mental retardation, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FKRP Lysate (NBL1-10743) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-10743

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Bioinformatics

Gene Symbol FKRP