FKBP38 Antibody - BSA Free

Western Blot: FKBP38 Antibody [NBP1-77909] - Shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.

Western blot using protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4 C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FKBP38 Antibody - BSA Free Summary

• Description: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads
  
  Store vial at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 ul). To minimize loss of volume dilute 1:10 by adding 225 ul of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.
• Immunogen: This protein A purified FKBP38 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP38 protein. (Uniprot: Q14318)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FKBP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000
  • Western Blot 1:1000
• Application Notes: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with FKBP38 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP38 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FKBP38 Antibody - BSA Free

• 38 kDa FK506-binding protein
• EC 5.2.1.8,38 kDa FKBP
• FK506 binding protein 8, 38kDa
• FK506-binding protein 8 (38kD)
• FK506-binding protein 8
• FKBP38
• FKBP-38
• FKBP38PPIase FKBP8
• FKBP8
• FKBP-8
• FKBPr38
• hFKBP38
• peptidyl-prolyl cis-trans isomerase FKBP8
• rotamase

Background

FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FKBP38 Antibody (NBP1-77909)(1)

Publication using NBP1-77909

• E Navarro-Vi, P de la Cruz, L Contreras, R González, M Negrete, MA Rodríguez-, LM Marín-Góme, JM Álamo-Mart, A Calvo, MA Gómez-Brav, J de la Cruz, J Padillo, J Muntané Molecular Pathways Leading to Induction of Cell Death and Anti-Proliferative Properties by Tacrolimus and mTOR Inhibitors in Liver Cancer Cells Cell. Physiol. Biochem., 2020-05-06;54(3):457-473. 2020-05-06 [PMID: 32369692]

Bioinformatics

• Gene Symbol: FKBP8
• Uniprot:
  • Q14318()
  • Q14318()
  • Q14318()
  • Q14318()
  • NP_036313(Human)