Recombinant Human FKBP13/FKBP2 Protein

SDS-Page: Recombinant Human FKBP13/FKBP2 Protein [NBP1-41233] - 13.4 kDa (122aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human FKBP13/FKBP2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 22-142 of Human FKBP13/FKBP2
  
  

  Source: E.coli

  Amino Acid Sequence: MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL

• Details of Functionality: Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FKBP2
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 13.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human FKBP13/FKBP2 Protein

• EC 5.2.1.8,13 kDa FK506-binding protein
• FK506 binding protein 2, 13kDa
• FK506-binding protein 2 (13kD)
• FK506-binding protein 2
• FKBP13
• FKBP13,13 kDa FKBP
• FKBP-13proline isomerase
• FKBP2
• FKBP-2
• Immunophilin FKBP13
• peptidyl-prolyl cis-trans isomerase FKBP2
• PPIase FKBP2
• PPIase
• rapamycin-binding protein
• rotamase

Background

FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Recombinant FKBP2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FKBP2