FKBP13/FKBP2 Overexpression Lysate (Native)

Images

 
Western Blot: FKBP2 Overexpression Lysate (Adult Normal) [NBL1-10736] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FKBP13/FKBP2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201608 and based on accession number NM_057092. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens FK506 binding protein 2, 13kDa (FKBP2), transcript variant 2, mRNA.
Gene
FKBP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FKBP13/FKBP2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FKBP13/FKBP2 Overexpression Lysate (Native)

  • EC 5.2.1.8,13 kDa FK506-binding protein
  • FK506 binding protein 2, 13kDa
  • FK506-binding protein 2 (13kD)
  • FK506-binding protein 2
  • FKBP13
  • FKBP13,13 kDa FKBP
  • FKBP-13proline isomerase
  • FKBP2
  • FKBP-2
  • Immunophilin FKBP13
  • peptidyl-prolyl cis-trans isomerase FKBP2
  • PPIase FKBP2
  • PPIase
  • rapamycin-binding protein
  • rotamase

Background

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-57062
Species: Hu
Applications: WB, ICC/IF
MAB3777
Species: Hu, Mu, Rt
Applications: WB
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
MAB7670
Species: Hu
Applications: WB, ICC
H00002288-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P, IP
MAB2294
Species: Hu, Mu
Applications: WB, IHC, IP
AF4094
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NB120-3562
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-19117
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-87929
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-97946
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-85360
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31348
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45861
Species: Hu
Applications: WB, Flow, IHC, IF
NBP2-58661
Species: Hu
Applications: IHC-P
NBP1-85358
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB600-1397
Species: Hu, Bv, Ca, Mk, Rb
Applications: WB, Flow, IHC, IHC-P, IP, MiAr, CyTOF-ready, Flow-IC
NBL1-10736
Species: Hu
Applications: WB

Publications for FKBP13/FKBP2 Lysate (NBL1-10736) (0)

There are no publications for FKBP13/FKBP2 Lysate (NBL1-10736).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FKBP13/FKBP2 Lysate (NBL1-10736) (0)

There are no reviews for FKBP13/FKBP2 Lysate (NBL1-10736). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FKBP13/FKBP2 Lysate (NBL1-10736) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FKBP13/FKBP2 Products

Bioinformatics Tool for FKBP13/FKBP2 Lysate (NBL1-10736)

Discover related pathways, diseases and genes to FKBP13/FKBP2 Lysate (NBL1-10736). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FKBP13/FKBP2 Lysate (NBL1-10736)

Discover more about diseases related to FKBP13/FKBP2 Lysate (NBL1-10736).
 

Pathways for FKBP13/FKBP2 Lysate (NBL1-10736)

View related products by pathway.

PTMs for FKBP13/FKBP2 Lysate (NBL1-10736)

Learn more about PTMs related to FKBP13/FKBP2 Lysate (NBL1-10736).

Blogs on FKBP13/FKBP2

There are no specific blogs for FKBP13/FKBP2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FKBP13/FKBP2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol FKBP2
Entrez