FKBP12.6 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: CoraFluor 1

FKBP12.6 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human FKBP12.6
  Met1-Glu108
  Accession # P68106
• Specificity: Detects human, mouse, and rat FKBP12.6 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human FKBP12 and less than 1% cross-reactivity with recombinant human FKBP13 and FKBP25 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FKBP1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for FKBP12.6 Antibody [CoraFluor™ 1]

• 12.6 kDa FK506-binding protein
• calstabin 2,12.6 kDa FKBP
• EC 5.2.1.8
• FK506 binding protein 1B, 12.6 kDa
• FK506-binding protein 12.6
• FK506-binding protein 1B (12.6 kD)
• FK506-binding protein 1B
• FKBP12.6
• FKBP-12.6
• FKBP12.6h-FKBP-12
• FKBP1B
• FKBP-1B
• FKBP1L
• FKBP9
• Immunophilin FKBP12.6
• OTK4
• OTK4PPIase FKBP1B
• peptidyl-prolyl cis-trans isomerase FKBP1B
• PKBP1L
• PPIase
• rotamase

Background

The protein encoded by the FKBP1B gene is a member of the immunophilin protein family, which play a role in immunoregulationand basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolylisomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A.Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are twoalternatively spliced transcript variants of this gene encoding different isoforms. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FKBP1B