Recombinant Human FKBP12 His Protein

SDS-Page: Recombinant Human FKBP12 Protein [NBC1-21651]

Product Discontinued

Recombinant Human FKBP12 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-108 of Human FKBP12
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE

• Details of Functionality: Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FKBP1A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 14.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human FKBP12 His Protein

• 12 kDa FKBP
• EC 5.2.1.8
• FK506 binding protein 1A, 12kDa
• FK506-binding protein 1A
• FKBP1
• FKBP12
• FKBP12C
• FKBP12FKBP12-Exip3
• FKBP-12T-cell, 12-kD
• FKBP1a
• FKBP-1A
• peptidyl-prolyl cis-trans isomerase FKBP1A
• PKC12
• PPIase FKBP1A
• PPIASE
• protein kinase C inhibitor 2
• rotamase

Background

FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for FKBP12 Recombinant Protein (NBC1-21651)(1)

Publication using NBC1-21651

• Zhang X, Crowley VM, Wucherpfennig TG Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16 Nat. Chem. Biol. 2019-07-01 [PMID: 31209349] (WB, Human)

Bioinformatics

• Gene Symbol: FKBP1A