HAF005). For additional reference, Recombinant Human FKBP12 (Catalog #
3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">
HAF005). For additional reference, Recombinant Human FKBP12 (Catalog #
3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #
HAF005). For additional reference, Recombinant Human FKBP12 (Catalog #
3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
![FKBP12 is decreased in PS19 mice brain.(A) Representative images showing the distribution of FKBP12 in entorhinal cortex of mouse brain. Scale bars, 50 μm. (B) Quantification of MAP-2 and FKBP12 fluorescence intensity in entorhinal cortex of PS19 mice brain in comparison to age-matched wild type (WT). Data are expressed as means ± SEM. N = 5. Statistics by unpaired t test, ***P < 0.005. (C) Representative Western blot showing high–molecular weight (HMW) tau aggregation in PS19 mice brain lysate and the decrease of FKBP12 in comparison to wild type. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (D) Quantification of the tau-5 band intensity showing the robust increase of the ratio between high–molecular weight tau to monomeric tau. (E) Quantification of the FKBP12 band intensity. (F) Representative Western blot images showing the expression level of phosphorylated tau (CP13 and PHF-1), FKBP12, and neuronal markers [postsynaptic density 95 (PSD-95) and MAP-2] in the brain lysate of 3-, 6-, and 9-month-old PS19 mice, respectively. (G) Quantification of the phosphorylated tau (CP13 and PHF-1) band intensity. Result was normalized by internal control of corresponding GAPDH band intensity. (H) Quantification of FKBP12 Western blot (WB) band intensity normalized by GAPDH and MAP-2, respectively. Statistics by one-way analysis of variance (ANOVA), post hoc multiple comparisons test by Tukey’s test. (I) Quantification for the band intensity of neuronal markers including PSD-95 and MAP-2, normalized by GAPDH. Data are expressed as means ± SEM. N = 3. Statistics by two-way ANOVA, and post hoc multiple comparisons test by Tukey’s. **P < 0.01, ***P < 0.005, and ****P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36724228), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab3777_human-mouse-rat-fkbp12-mab-clone-422513-immunohistochemistry-1212202585781.jpg)
![FKBP12 is decreased in PS19 mice brain.(A) Representative images showing the distribution of FKBP12 in entorhinal cortex of mouse brain. Scale bars, 50 μm. (B) Quantification of MAP-2 and FKBP12 fluorescence intensity in entorhinal cortex of PS19 mice brain in comparison to age-matched wild type (WT). Data are expressed as means ± SEM. N = 5. Statistics by unpaired t test, ***P < 0.005. (C) Representative Western blot showing high–molecular weight (HMW) tau aggregation in PS19 mice brain lysate and the decrease of FKBP12 in comparison to wild type. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (D) Quantification of the tau-5 band intensity showing the robust increase of the ratio between high–molecular weight tau to monomeric tau. (E) Quantification of the FKBP12 band intensity. (F) Representative Western blot images showing the expression level of phosphorylated tau (CP13 and PHF-1), FKBP12, and neuronal markers [postsynaptic density 95 (PSD-95) and MAP-2] in the brain lysate of 3-, 6-, and 9-month-old PS19 mice, respectively. (G) Quantification of the phosphorylated tau (CP13 and PHF-1) band intensity. Result was normalized by internal control of corresponding GAPDH band intensity. (H) Quantification of FKBP12 Western blot (WB) band intensity normalized by GAPDH and MAP-2, respectively. Statistics by one-way analysis of variance (ANOVA), post hoc multiple comparisons test by Tukey’s test. (I) Quantification for the band intensity of neuronal markers including PSD-95 and MAP-2, normalized by GAPDH. Data are expressed as means ± SEM. N = 3. Statistics by two-way ANOVA, and post hoc multiple comparisons test by Tukey’s. **P < 0.01, ***P < 0.005, and ****P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36724228), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab3777_human-mouse-rat-fkbp12-mab-clone-422513-immunohistochemistry-121220259181919.jpg)
![Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Western-Blot-NBP2-02093-img0002.jpg "Western Blot: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.")
![Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] Staining of paraffin-embedded Human Kidney tissue using anti-FKBP12 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Immunohistochemistry-Paraffin-NBP2-02093-img0004.jpg)
![Flow Cytometry: FKBP12 Antibody (3B3) [NBP2-02093] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FKBP12 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Flow-Cytometry-NBP2-02093-img0001.jpg)
![Immunohistochemistry-Paraffin: FKBP12 Antibody (3B3) [NBP2-02093] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBP12 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FKBP12-Antibody-3B3-Immunohistochemistry-Paraffin-NBP2-02093-img0003.jpg)
![Western Blot: FKBP12 Antibody [NB300-508] - Analysis of purified FKBP12 after immunoprecipatation on a 15% gel.](http://images.novusbio.com/fullsize/FKBP12-Antibody-Western-Blot-NB300-508-img0009.jpg "Western Blot: FKBP12 Antibody [NB300-508] - Analysis of purified FKBP12 after immunoprecipatation on a 15% gel.")
![Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Colon carcinoma tissue.](http://images.novusbio.com/fullsize/FKBP12-Antibody-Immunohistochemistry-Paraffin-NB300-508-img0008.jpg)
![Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Heart tissue.](http://images.novusbio.com/fullsize/FKBP12-Antibody-Immunohistochemistry-Paraffin-NB300-508-img0003.jpg)
![Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/FKBP12-Antibody-Immunohistochemistry-Paraffin-NB300-508-img0006.jpg)
![Western Blot: FKBP12 Antibody [NBP2-41272] - Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/FKBP12-Antibody-Western-Blot-NBP2-41272-img0001.jpg "Western Blot: FKBP12 Antibody [NBP2-41272] - Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] - Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41272_rabbit-polyclonal-fkbp12-antibody-271120241937714.jpg)
![Immunocytochemistry/ Immunofluorescence: FKBP12 Antibody - BSA Free [NBP2-41272] - Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41272_rabbit-polyclonal-fkbp12-antibody-271120242011559.jpg)
![Western Blot: FKBP12 Antibody (1E5-A12) [H00002280-M01] - Analysis of FKBP1A expression in transfected 293T cell line by FKBP1A monoclonal antibody (M01), clone 1E5-A12.Lane 1: FKBP1A transfected lysate(12 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FKBP12-Antibody-1E5-A12-Western-Blot-H00002280-M01-img0005.jpg "Western Blot: FKBP12 Antibody (1E5-A12) [H00002280-M01] - Analysis of FKBP1A expression in transfected 293T cell line by FKBP1A monoclonal antibody (M01), clone 1E5-A12.Lane 1: FKBP1A transfected lysate(12 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: FKBP12 Antibody (1E5-A12) [H00002280-M01] - FKBP1A monoclonal antibody (M01), clone 1E5-A12 Analysis of FKBP1A expression in HL-60.](http://images.novusbio.com/fullsize/FKBP12-Antibody-1E5-A12-Western-Blot-H00002280-M01-img0004.jpg)
![Sandwich ELISA: FKBP12 Antibody (1E5-A12) [H00002280-M01] - Detection limit for recombinant GST tagged FKBP1A is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FKBP12-Antibody-1E5-A12-Sandwich-ELISA-H00002280-M01-img0003.jpg)
![Western Blot: FKBP12 Antibody (E3) [NBP3-41645] - Sample: Recombinant FKBP12, Human.](http://images.novusbio.com/fullsize/nbp3-41645_mouse-fkbp12-mab-e3-612202421262979.jpg "Western Blot: FKBP12 Antibody (E3) [NBP3-41645] - Sample: Recombinant FKBP12, Human.")
![Immunohistochemistry-Paraffin: FKBP12 Antibody (E3) [NBP3-41645] - DAB staining on IHC-P; Samples: Human Heart Tissue; Primary Ab: 10ug/ml NBP3-41645; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41645_mouse-fkbp12-mab-e3-61220242151852.jpg)
![Immunohistochemistry-Paraffin: FKBP12 Antibody (E3) [NBP3-41645] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41645; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41645_mouse-fkbp12-mab-e3-612202421351246.jpg)
![Western Blot: FKBP12 Antibody (9A2J5) [NBP3-15418] - Western blot analysis of extracts of various cell lines, using FKBP12 Rabbit mAb (NBP3-15418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FKBP12-Antibody-9A2J5-Western-Blot-NBP3-15418-img0001.jpg "Western Blot: FKBP12 Antibody (9A2J5) [NBP3-15418] - Western blot analysis of extracts of various cell lines, using FKBP12 Rabbit mAb (NBP3-15418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: FKBP12 Antibody (9A2J5) [NBP3-15418] - Western blot analysis of extracts of U-251MG cells, using FKBP12 Rabbit mAb (NBP3-15418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/FKBP12-Antibody-9A2J5-Western-Blot-NBP3-15418-img0002.jpg)
![Western Blot: FKBP12 Overexpression Lysate (Adult Normal) [NBL1-10732] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP12.](http://images.novusbio.com/fullsize/FKBP12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10732-img0002.jpg "Western Blot: FKBP12 Overexpression Lysate (Adult Normal) [NBL1-10732] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP12.")
![Western Blot: FKBP12 Overexpression Lysate (Adult Normal) [NBL1-10733] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP12.](http://images.novusbio.com/fullsize/FKBP12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10733-img0002.jpg "Western Blot: FKBP12 Overexpression Lysate (Adult Normal) [NBL1-10733] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FKBP12.")
![Western Blot: FKBP12 Antibody [NBP2-97584] - Anti-FKBP12 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: SH-SY5Y Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: U937 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 13 kDa](http://images.novusbio.com/fullsize/FKBP12-Antibody-Western-Blot-NBP2-97584-img0002.jpg "Western Blot: FKBP12 Antibody [NBP2-97584] - Anti-FKBP12 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: SH-SY5Y Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: U937 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 13 kDa")
![Western Blot: FKBP12 Antibody [NBP2-99149] - Anti-FKBP12 rabbit polyclonal antibody at 1:500 dilution. Lane A: SH-SY5Y Whole Cell Lysate. Lane B: HL-60 Whole Cell Lysate. Lane C: U937 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 12 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/FKBP12-Antibody-Western-Blot-NBP2-99149-img0002.jpg "Western Blot: FKBP12 Antibody [NBP2-99149] - Anti-FKBP12 rabbit polyclonal antibody at 1:500 dilution. Lane A: SH-SY5Y Whole Cell Lysate. Lane B: HL-60 Whole Cell Lysate. Lane C: U937 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 12 kDa (We are unsure as to the identity of these extra bands).")
