Filamin Antibody (FLMN01 (PM6/317))

Western Blot: Filamin Antibody (FLMN01 (PM6/317)) [NB600-1218] - Analysis of 7.5ug of HeLa cell lysate per well.

Immunohistochemistry-Paraffin: Filamin Antibody (FLMN01 (PM6/317)) [NB600-1218] - Staining of human skin stained with Filamin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscle cells surrounding blood vessels.

Western Blot: Filamin Antibody (FLMN01 (PM6/317)) [NB600-1218] - Analysis of NIH3T3 Cells.

Product Discontinued

Filamin Antibody (FLMN01 (PM6/317)) Summary

• Immunogen: Platelet filamin
• Localization: Cytoplasm; cell cortex
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLNC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Western Blot 1:100 - 1:500
• Control:

  NIH 3T3 Whole Cell Lysate
  Human Skin Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for Filamin Antibody (FLMN01 (PM6/317))

• ABP-280
• ABP280A
• ABP-280-like protein
• ABPA
• ABP-L
• ABPLABP-L, gamma filamin
• Actin-binding-like protein
• filamin 2
• filamin C, gamma (actin binding protein 280)
• filamin C, gamma
• filamin-2
• filamin-C
• FLJ10186
• FLN2actin binding protein 280
• FLNc
• FLN-C
• Gamma-filamin

Background

Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Filamin Antibody (NB600-1218)(1)

Publication using NB600-1218

• Akcan M Defining the role of Xin and associated proteins in skeletal muscle health Thesis 1905-07-13 (WB, Mouse)

FAQs for Filamin Antibody (NB600-1218). (Showing 1 - 1 of 1 FAQ).

1. Which region is the Filamin Antibody (FLMN01 (PM6/317)) directed, N-terminal or C-terminal?
   • NB600-1218 was made to the full-length human filamin protein. Unfortunately, we have not epitope mapped this antibody, so we do not know the specific region targeted. This is a licensed clone and all information comes directly from the inventor of the clone. We recommend doing a publication search on the clone number/name to see if any further epitope information is available.

Bioinformatics

• Gene Symbol: FLNC
• Entrez:
  • 2318(Human)
  • 68794(Mouse)
  • 362332(Rat)
• Uniprot:
  • P21333(Human)