Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - FAP monoclonal antibody (M01), clone 1E5 Analysis of FAP expression in HepG2.

Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAP MaxPab rabbit polyclonal antibody.

ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Detection limit for recombinant GST tagged FAP is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FAP (AAH26250, 525 a.a. ~ 624 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWS
• Specificity: FAP - fibroblast activation protein, alpha
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody (1E5) - Azide and BSA Free

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Activation Protein alpha/FAP Antibody (H00002191-M01)(6)

Publications using H00002191-M01

• R Katsube, K Noma, T Ohara, N Nishiwaki, T Kobayashi, S Komoto, H Sato, H Kashima, T Kato, S Kikuchi, H Tazawa, S Kagawa, Y Shirakawa, H Kobayashi, T Fujiwara Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer Scientific Reports, 2021-01-18;11(1):1693. 2021-01-18 [PMID: 33462372]
• Kashima H, Noma K, Ohara T et al. Cancer-associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma. Int J Cancer. 2018-12-03 [PMID: 30367467]
• Chung KM, Hsu SC, Chu YR et al. Fibroblast Activation Protein (FAP) Is Essential for the Migration of Bone Marrow Mesenchymal Stem Cells through RhoA Activation. PLoS One. 2014-02-13 [PMID: 24551161]
• Bohonowych J, Hance M, Nolan K et al. Extracellular Hsp90 mediates an NF-kappa dependent inflammatory stromal program: Implications for the prostate tumor microenvironment. Prostate 2014-04-01 [PMID: 24338924] (Human)
• Denys H, Derycke L, Hendrix A et al. Differential impact of TGF-beta EGF on fibroblast differentiation invasion reciprocally promotes colon cancer cell invasion. Cancer Lett;266(2):263-74. 2008-08-08 [PMID: 18423981]
• Wang XM, Yao TW, Nadvi NA et al. Fibroblast activation protein chronic liver disease. Front Biosci;13:3168-80. 2008-01-01 [PMID: 17981786]

Review for Fibroblast Activation Protein alpha/FAP Antibody (H00002191-M01) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Christian Zuppinger WB Human 07/14/2016

Summary

• Application: Western Blot
• Sample Tested: lysates of 2D standard cultures of primary cardiac fibroblasts, RIPA or Laemmli lysate buffer was used
• Species: Human
• Lot: F4281-1E5

Bioinformatics

• Gene Symbol: FAP
• Entrez:
  • 2191(Human)
• OMIM:
  • 600403(Human)
• Uniprot:
  • AAH26250(Human)