Fibrinopeptide A Antibody (1F7)

Product Discontinued

Fibrinopeptide A Antibody (1F7) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fibrinopeptide A conjugated with a carrier protein.
• Specificity: Fibrinopeptide A region in the alpha-chain of fibrinogen and free fibrinopeptide A.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Fibrinogen and fibrinopeptide A immunoassay. For specific fibrinogen detection in sandwich ELISA we recommend using anti-fibrinopeptide A MAbs for coating and anti-fibrinogen MAbs for conjugation. Recommended pairs (capture-detection): NB100-73041 - NB120-10066

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Fibrinopeptide A Antibody (1F7)

• FGA
• Fib2
• Fibrinogen A
• Fibrinogen alpha chain
• Fibrinogen alpha subunit
• Fibrinogen Type I
• fibrinogen, A alpha polypeptide
• FPA
• MGC119422
• MGC119423
• MGC119425

Background

Fibrinogen is the main protein of the blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A and B are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerisation forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Fibrinopeptide A Antibody (NB100-73041). (Showing 1 - 2 of 2 FAQ).

1. Have you tested this product to determine reactivity to fibrinogen and fibrin? Can you email me the results? Before ordering we need proof this antibody reacts only to fibrinogen and not to fibrin.
   • I do not see any specific notes or testing data on file from the lab indicating direct testing for reactivity to fibrin.
2. So this is a product with no negative control? Hasn't someone at least tested it on tissue such as skin from someone with active angiogenesis to make sure it doesn't bind clots?
   • I heard back from the lab this morning and they had indicated that unfortunately we can't say for sure that our anti-fibrinopeptide A antibody will not detect fibrin. It was believed for a long time that fibrinopeptide A is present only in fibrinogen and fibrin did not contain it when this antibody was synthesized. The latest data shows however, that fibrin contains a significant amount of uncleaved fibrinopeptide A that is whole uncleaved fibrinogen molecules are incorporated into fibrin. As a result we do not have any direct testing data on file that we can send to show with certainty that it will or will not detect fibrin.

Bioinformatics

• Gene Symbol: FGA
• Entrez:
  • 2243(Human)
• Uniprot:
  • P02671()