Rat Fibrinogen ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Fibrinogen (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat fibrinogen. Suitable Sample: Cell Culture Supernatant, Urine Sample Volume: 50 uL |
| Standard Curve Range |
3.125 to 200 ng/mL |
| Sensitivity |
2 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 7.90% |
| Intra-Assay |
CV% < 5.10% |
| Spike Recovery |
95.00% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
FGA |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Rat FBG Antibody (50x): A 50-fold concentrated biotinylated polyclonalantibody against Rat FBG.
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine
- FBG Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with apolyclonal antibody against FBG.
- FBG Standard” Rat FBG in a buffered protein base.
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
- Sealing Tapes: pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
- Stop Solution: A 0.5 N hydroxychloric acid to stop the chromogen substrate reaction
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Rat Fibrinogen ELISA Kit (Colorimetric)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Fibrinogen ELISA Kit (KA0505). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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