FCRL3/FcRH3 Antibody (546828) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clone: 546828
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

FCRL3/FcRH3 Antibody (546828) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human FCRL3/FcRH3 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FCRL2 and rhFCRL5 is observed, and no cross-reactivity with rhFCRL1 or recombinant mouse FCRL3 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL3/FcRH3 Antibody (546828) [Alexa Fluor™ Plus 680]

• CD307c
• Fc receptor homolog 3
• Fc receptor-like 3
• Fc receptor-like protein 3
• FcRH3
• FCRL3
• fcR-like protein 3
• hIFGP3
• IFGP3
• immunoglobulin superfamily receptor translocation associated protein 3
• IRTA3
• SPAP2

Background

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2-4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
2. Miller, I. et al. (2002) Blood, 99:2662.
3. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
4. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
5. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
6. Kochi, Y. et al. (2005) Nat. Genet. 37:478.
7. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.