FBXO45 Antibody

Immunocytochemistry/Immunofluorescence: FBXO45 Antibody [NBP1-91891] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: FBXO45 Antibody [NBP1-91891] - Staining of human thyroid gland shows strong cytoplasmic and nuclear positivity in glandular cells.

Product Discontinued

FBXO45 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VIGIATKRAPMQCQGYVALLGSDDQSWGWNLVDNNLLHNGEVNGSFPQCNNAPKYQIGERIRVILDMEDKTLAFERGYEFLGVAFRGLPKVCL
• Predicted Species: Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXO45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in WB reported in scientific literature (PMID:31285543).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FBXO45 Antibody

• F-box only protein 45
• F-box protein 45
• F-box/SPRY domain-containing protein 1
• Fbx45

Background

F-box/SPRY domain-containing protein 1, also known as F-box only protein 45 and FBXO45, is a component of the E3 ubiquitin ligase complex. FBXO45 is required for normal neuromuscular synaptogenesis, neuronal migration and axon pathfinding. FBXO45 also plays a role in regulating neurotransmission by mature neurons and controlling synaptic activity via UNC13A in the ubiquitin dependent pathway. FBXO45 protein recognizes TP73 and promotes it ubiquitination and degradation. FBXO45 expression is down regulated in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXO45 Antibody (NBP1-91891)(1)

Publication using NBP1-91891

• Richter KT, Kschonsak YT, Vodicska B, Hoffmann I FBXO45-MYCBP2 regulates mitotic cell fate by targeting FBXW7 for degradation Cell Death Differ. 2020-02-01 [PMID: 31285543] (WB, Human)

Bioinformatics

• Gene Symbol: FBXO45
• Entrez:
  • 200933(Human)
• Uniprot:
  • P0C2W1(Human)