FBXL4 Antibody - BSA Free

Western Blot: FBXL4 Antibody [NBP2-16441] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human FBXL4-transfected 293T cells 7.5 % SDS-PAGE FBL4 antibody [N1C1] dilution: 1:1000

Immunocytochemistry/Immunofluorescence: FBXL4 Antibody [NBP2-16441] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: FBXL4 Antibody [NBP2-16441] - BT474 xenograft, using FBL4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Po, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FBXL4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human FBXL4. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Mouse (91%), Porcine (92%), Rhesus Macaque (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXL4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for FBXL4 Antibody - BSA Free

• FBL5F-box/LRR-repeat protein 4
• F-box and leucine-rich repeat protein 4FBL4F-box protein FBL4
• F-box protein FBL4/FBL5

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 9 tandem leucine-rich repeats. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for FBXL4 Antibody (NBP2-16441). (Showing 1 - 1 of 1 FAQ).

1. Could you please provide me with the epitope sequence of the following antibody: FBXL4 Antibody (NBP2-16441)?
   • Due to proprietary issues, the exact epitope sequence of NBP2-16441 can not be disclosed, however, the immunogen corresponds to a region within amino acids 43 and 351 of FBL4 (Uniprot ID#Q9UKA2).

Bioinformatics

• Gene Symbol: FBXL4
• Uniprot:
  • Q9UKA2(Human)