FARSLA Antibody - BSA Free

Western Blot: FARSLA Antibody [NBP2-97761] - Anti-FARSLA rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: FARSLA Antibody [NBP2-97761] - FARSLA was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-FARSLA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FARSLA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa

Immunocytochemistry/Immunofluorescence: FARSLA Antibody [NBP2-97761] - Immunofluorescence staining of FARSLA in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97761 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FARSLA Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human FARSLA fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FARSA
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for FARSLA Antibody - BSA Free

• CML33pheRS
• EC 6.1.1.20
• FARS
• FARSL
• FARSLA
• FRSA
• PheHA
• phenylalanine tRNA ligase 1, alpha, cytoplasmic
• Phenylalanine--tRNA ligase alpha chain
• phenylalanine-tRNA synthetase alpha-subunit
• phenylalanine-tRNA synthetase-like
• phenylalanine-tRNA synthetase-like, alpha subunit
• phenylalanyl-tRNA synthetase alpha chain
• phenylalanyl-tRNA synthetase, alpha subunit
• phenylalanyl-tRNA synthetase-like, alpha subunit
• PheRS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FARSA