FAK [p Tyr397] Antibody (820755)

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 820755
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

FAK [p Tyr397] Antibody (820755) Summary

• Immunogen: Phosphopeptide containing the human FAK Y397 site
  Accession # Q05397
• Modification: p Tyr397
• Specificity: Detects human FAK when phosphorylated at Y397.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PTK2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FAK [p Tyr397] Antibody (820755)

• EC 2.7.10
• FADK 1
• FADK1
• FADKFAK-related non-kinase polypeptide
• FAK
• FAK1FRNK
• FAKEC 2.7.10.2
• focal adhesion kinase 1
• pp125FAK
• Protein-tyrosine kinase 2
• PTK2 protein tyrosine kinase 2
• PTK2

Background

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAK Antibody (MAB4528)(4)

Publications using MAB4528

• K Wantoch vo, P König, S Henze, M Schlesinge, P Zawierucha, R Januchowsk, G Bendas Insight into Cisplatin-Resistance Signaling of W1 Ovarian Cancer Cells Emerges mTOR and HSP27 as Targets for Sensitization Strategies International Journal of Molecular Sciences, 2020-12-03;21(23):. 2020-12-03 [PMID: 33287446] (Western Blot, Human)
• Kyuho Jeong, James M. Murphy, Yelitza A.R. Rodriguez, Jun-Sub Kim, Eun-Young Erin Ahn, Ssang-Taek Steve Lim FAK inhibition reduces metastasis of alpha 4 integrin-expressing melanoma to lymph nodes by targeting lymphatic VCAM-1 expression Biochemical and Biophysical Research Communications 2019-02-01 [PMID: 30660359]
• JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-03-08;0(0):. 2017-03-08 [PMID: 28286026] (Western Blot, Human)
• Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013-03-27;87(11):6326-35. 2013-03-27 [PMID: 23536671] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PTK2
• Uniprot:
  • Q05397(Human)