FABP8/M-FABP/Myelin P2 Protein Antibody (631306) [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human FABP8/M-FABP Monoclonal Antibody (Catalog # MAB5866) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FABP8/M-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FABP8/M-FABP was detected in immersion fixed paraffin-embedded sections of human spinal cord using using Mouse Anti-Human FABP8/ M-FABP Mono-clonal Antibody (Catalog # MAB5866) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to white matter. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 631306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FABP8/M-FABP/Myelin P2 Protein Antibody (631306) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FABP8/M‑FABP
  Ser2-Val132
  Accession # P02689
• Specificity: Detects human FABP8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 5, 6, 7, or 9 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP8/M-FABP/Myelin P2 Protein Antibody (631306) [Unconjugated]

• FABP8
• MFABP
• M-FABP
• MP2
• myelin P2 protein
• P2
• peripheral myelin protein 2FABP8
• PMP2

Background

FABP8 (fatty acid binding protein-8; also M [myelin]-FABP, P2 and PMP2) is a 15 kDa (predicted) member of the fatty acid binding protein family, calycin superfamily of molecules. It is found in Schwann cells, presumably on the cytoplasmic face of the plasma membrane where it may contribute to fatty acid transport across myelin. Functionally, FABP8 has a high affinity for U-shaped fatty acids such as oleic and palmitic acid. Human FABP8 is 132 amino acids (aa) in length and exhibits two layers of antiparallel beta -strands that envelope a hydrophobic pocket for lipid binding. Arg107 plus Arg127-Ile128-Tyr129 participate in fatty acid binding. Full length human FABP8 shares 87% and 95% aa identity with mouse and rabbit FABP9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMP2
• Uniprot:
  • P02689(Human)