Exonuclease 1 Antibody

Product Discontinued

Exonuclease 1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 55-05 of Human Exo1.
• Specificity: Exo1 (K86) pAb detects endogenous levels of Exo1 rotein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Exo1 (K86) pAb in extracts from raw264.7 cells. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24591601)
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Exonuclease 1 Antibody

• EXOI
• exonuclease 1
• Exonuclease I
• HEX1rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
• hExo1
• hExoIEC 3.1

Background

Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the mExo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/ hEXO1 gene to chromosome 1q42-q43. mExo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The mismatch repair (MMR) system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MM. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Exonuclease 1 Antibody (NBP1-19709)(4)

Publications using NBP1-19709

• Zahn A, Eranki AK, Patenaude AM et al. Activation induced deaminase C-terminal domain links DNA breaks to end protection and repair during class switch recombination. Proc. Natl. Acad. Sci. U.S.A. 2014-03-03 [PMID: 24591601] (Chemotaxis, Mouse)
• Cortizas EM, Zahn A, Hajjar ME et al. Alternative End-Joining and Classical Nonhomologous End-Joining Pathways Repair Different Types of Double-Strand Breaks during Class-Switch Recombination. J Immunol. 2013-12-01 [PMID: 24146042] (WB, Mouse)
• Peterson SE, Li Y, Chait BT et al. Cdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair. J Cell Biol;194(5):705-720. 2011-09-05 [PMID: 21893598]
• van Dam TP DNA polymerase beta prevents AID-instigated mutagenic non-canonical mismatch DNA repair bioRxiv 2020-01-01 (IP, Mouse)

Bioinformatics

• Gene Symbol: EXO1
• Entrez:
  • 9156(Human)
  • 26909(Mouse)