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ETS2 Overexpression Lysate

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Western Blot: ETS2 Overexpression Lysate (Adult Normal) [NBL1-10356] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ETS2.
Western Blot: ETS2 Overexpression Lysate (Adult Normal) [NBL1-10356] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ETS2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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ETS2 Overexpression Lysate Summary

Description

ETS2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207377

Accession#: NM_005239

Protein Tag: C-MYC/DDK
Gene
ETS2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
52.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ETS2 Overexpression Lysate

  • ETS2IT1
  • human erythroblastosis virus oncogene homolog 210protein C-ets-2
  • oncogene ETS-2
  • v-ets avian erythroblastosis virus E2 oncogene homolog 2
  • v-ets avian erythroblastosis virus E26 oncogene homolog 2
  • v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Background

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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ETS2
Overexpression Lysate
NBL1-10356
ETS2 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol ETS2