ERp57/PDIA3 Antibody

Images

 
Western Blot: ERp57/PDIA3 Antibody [NB100-1143] - Detection of ERp57 in A549 cell lysate (RIPA buffer, 35 ug total protein per lane) using NB100-1143 (1 ug/ml). Detected by western blot using chemiluminescence.
Western Blot: ERp57/PDIA3 Antibody [NB100-1143] - (1ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ERp57/PDIA3 Antibody Summary

Immunogen
Peptide with sequence C-EEKPKKKKKAQEDL corresponding to C-Terminus according to NP_005304.3.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDIA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 55 kDa band observed in lysates of HepG2 and NIH3T3 (calculated MW of 56.8 kDa band according to NP_005304.3).
Positive Control
ERp57/PDIA3 Lysate (NBL1-14244)
Publications
Read Publication using NB100-1143.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERp57/PDIA3 Antibody

  • Disulfide isomerase ER-60
  • EC 5.3.4.1
  • endoplasmic reticulum P58
  • Endoplasmic reticulum resident protein 57
  • Endoplasmic reticulum resident protein 60
  • ER protein 60
  • ER60
  • ERp57
  • ERp5758 kDa glucose-regulated protein
  • ERp60
  • ERp6058 kDa microsomal protein
  • ERp61
  • glucose regulated protein, 58kDa
  • GRP57
  • GRP58
  • GRP58ER protein 57
  • HsT17083
  • P58
  • PDIA3
  • phospholipase C-alpha
  • PI-PLC
  • protein disulfide isomerase family A, member 3
  • protein disulfide isomerase-associated 3
  • protein disulfide-isomerase A3

Background

ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ERp57/PDIA3 Antibody (NB100-1143)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDIA3
Entrez
Uniprot