ERp57/PDIA3 Antibody

Product Discontinued

ERp57/PDIA3 Antibody Summary

• Immunogen: Peptide with sequence C-EEKPKKKKKAQEDL corresponding to C-Terminus according to NP_005304.3.
• Localization: Endoplasmic reticulum
• Marker: Endoplasmic Reticulum Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDIA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 0.3 ug/mL
• Control:

  ERp57/PDIA3 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence similarity: Rat, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERp57/PDIA3 Antibody

• Disulfide isomerase ER-60
• EC 5.3.4.1
• endoplasmic reticulum P58
• Endoplasmic reticulum resident protein 57
• Endoplasmic reticulum resident protein 60
• ER protein 60
• ER60
• ERp57
• ERp5758 kDa glucose-regulated protein
• ERp60
• ERp6058 kDa microsomal protein
• ERp61
• glucose regulated protein, 58kDa
• GRP57
• GRP58
• GRP58ER protein 57
• HsT17083
• P58
• PDIA3
• phospholipase C-alpha
• PI-PLC
• protein disulfide isomerase family A, member 3
• protein disulfide isomerase-associated 3
• protein disulfide-isomerase A3

Background

ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERp57/PDIA3 Antibody (NB100-1143)(1)

Publication using NB100-1143

• Koivunen P, Horelli-Kuitunen N, Helaakoski T et al. Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21. Genomics 1997-06-15 [PMID: 9205111]

Bioinformatics

• Gene Symbol: PDIA3
• Entrez:
  • 2923(Human)
  • 14827(Mouse)
  • 29468(Rat)
• Uniprot:
  • NP_005304.(Human)