ERManI Antibody - Azide and BSA Free

Western Blot: ERManI Antibody [NBP3-03426] - Analysis of extracts of various cell lines, using ERManI antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Product Discontinued

ERManI Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ERManI (NP_057303.2). MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAN1B1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ERManI Antibody - Azide and BSA Free

• alpha 1,2-mannosidase
• EC 3.2.1.113
• endoplasmic reticulum alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• ER alpha 1,2-mannosidase
• ER alpha-1,2-mannosidase
• ER ManI
• ER mannosidase 1
• ERMan1
• Man9GlcNAc2-specific processing alpha-mannosidase
• Man9GlcNAc2-specific-processing alpha-mannosidase
• MANA-ER
• Mannosidase alpha class 1B member 1
• mannosidase, alpha, class 1B, member 1
• MRT15
• RP11-350O14.2

Background

ERManI (MAN1B1) catalyzes the first mannose-trimming step in maturation of Asn-linked oligosaccharides. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), specifically converting it to Man8GlcNAc isomer B. ERManI may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MAN1B1