ERManI Antibody (3C2) [DyLight 650]

• Reactivity: Hu, Mu(-)
• Applications: WB, ELISA, ICC/IF, IHC, IP, KO
• Clone: 3C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

ERManI Antibody (3C2) [DyLight 650] Summary

• Immunogen: Full length human recombinant ERManI [Swiss-Prot# Q9UKM7]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAN1B1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for ERManI Antibody (3C2) [DyLight 650]

• alpha 1,2-mannosidase
• EC 3.2.1.113
• endoplasmic reticulum alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
• endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• ER alpha 1,2-mannosidase
• ER alpha-1,2-mannosidase
• ER ManI
• ER mannosidase 1
• ERMan1
• Man9GlcNAc2-specific processing alpha-mannosidase
• Man9GlcNAc2-specific-processing alpha-mannosidase
• MANA-ER
• Mannosidase alpha class 1B member 1
• mannosidase, alpha, class 1B, member 1
• MRT15
• RP11-350O14.2

Background

ERManI, also known as endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase or MAN1B1, is a putative ER resident mannosidase that plays a rate-limiting role in generating a signal that targets misfolded N-linked glycoproteins for ER-associated degradation (ERAD). ERManI codes for an enzyme that converts Man9GlcNAc to Man8GlcNAc isomer B during N-glycan biosynthesis. It also facilitates the trimming of Man5-6GlcNAc2 in the endoplasmic reticulum associated degradation pathway. Multiple transcript variants of ERManI can result from alternative splicing, and a related pseudogene can be found on chromosome 11. Research has found that mutations on the ERManI gene can result in autosomal-recessive intellectual disabilities and developmental delays (PMID: 21763484). ERManI is predominately localized to the Golgi and endoplasmic reticulum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAN1B1