ERManI Antibody (3C2) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
3C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

ERManI Antibody (3C2) [Alexa Fluor® 488] Summary

Immunogen
Full length human recombinant ERManI [Swiss-Prot# Q9UKM7]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAN1B1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This ERManI Antibody (3C2) is useful for Immunocytochemistry/Immunofluorescence, Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry on paraffin embedded sections. In ICC/IF, endoplasmic reticulum membrane staining was observed in HeLa cells. In Western Blot, a band is seen ~90 kDa representing ERManI. In IHC-P, staining was observed in the endoplasmic reticulum of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human. Does not cross react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ERManI Antibody (3C2) [Alexa Fluor® 488]

  • alpha 1,2-mannosidase
  • EC 3.2.1.113
  • endoplasmic reticulum alpha-mannosidase 1
  • endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
  • endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
  • ER alpha 1,2-mannosidase
  • ER alpha-1,2-mannosidase
  • ER ManI
  • ER mannosidase 1
  • ERMan1
  • Man9GlcNAc2-specific processing alpha-mannosidase
  • Man9GlcNAc2-specific-processing alpha-mannosidase
  • MANA-ER
  • Mannosidase alpha class 1B member 1
  • mannosidase, alpha, class 1B, member 1
  • MRT15
  • RP11-350O14.2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERManI Antibody (NBP2-13167AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP2-13167AF647
Alexa Fluor 700 Labeled NBP2-13167AF700

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Bioinformatics Tool for ERManI Antibody (NBP2-13167AF488)

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Bioinformatics

Gene Symbol MAN1B1