ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of monoclonal antibody to MAPK6 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPK6 (AAH35492, 612 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
• Specificity: MAPK6 - mitogen-activated protein kinase 6
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERK3/MAPK6 Antibody (1G6) - Azide and BSA Free

• DKFZp686F03189
• EC 2.7.11
• EC 2.7.11.24
• ERK3
• ERK-3
• ERK3p97-MAPK
• Extracellular signal-regulated kinase 3
• extracellular signal-regulated kinase, p97
• HsT17250
• MAP kinase 6
• MAP kinase isoform p97
• MAPK 6
• MAPK6
• mitogen-activated protein kinase 6
• p97MAPK
• PRKM6
• protein kinase, mitogen-activated 5
• protein kinase, mitogen-activated 6

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK6
• Entrez:
  • 5597(Human)
• OMIM:
  • 602904(Human)
• Uniprot:
  • AAH35492(Human)