ERCC1 Antibody (JM10-07)

Images

 
Western Blot: ERCC1 Antibody (JM10-07) [NBP2-66823] - Analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human lung Lane 3: 293T Lane 4: A549
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (JM10-07) [NBP2-66823] - Staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: ERCC1 Antibody (JM10-07) [NBP2-66823] - Analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (JM10-07) [NBP2-66823] - Staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (JM10-07) [NBP2-66823] - Staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (JM10-07) [NBP2-66823] - Staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (JM10-07) [NBP2-66823] - Staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more

Product Details

Summary
Product Discontinued
View other related ERCC1 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-66823
    • Availability
      Product Discontinued

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ERCC1 Antibody (JM10-07) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human ERCC1 aa 1-220 / 297. (SwissProt: P07992 Human; SwissProt: P07903 Mouse)
Localization
Cytoplasm, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ERCC1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ERCC1 Antibody (JM10-07)

  • COFS4
  • DNA excision repair protein ERCC-1
  • ERCC1
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence)
  • RAD10
  • UV20

Background

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ERCC1 Antibody (NBP2-66823) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERCC1 Antibody (NBP2-66823) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ERCC1 Products

Research Areas for ERCC1 Antibody (NBP2-66823)

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Blogs on ERCC1.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.

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Bioinformatics

Gene Symbol ERCC1