ErbB3/Her3 Antibody (66219) [Allophycocyanin]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 66219
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

ErbB3/Her3 Antibody (66219) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ErbB3
  Ser20-Thr643
  Accession # P21860
• Specificity: Detects human ErbB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant human (rh) EGF R, rhErbB4, or rhErbB2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERBB3
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from ascites

Alternate Names for ErbB3/Her3 Antibody (66219) [Allophycocyanin]

• c-erbB3
• EC 2.7.10
• EC 2.7.10.1
• ErbB3
• ErbB-3
• erbB3-S
• HER3
• HER3c-erbB-3
• LCCS2
• lethal congenital contracture syndrome 2
• MDA-BF-1
• MGC88033
• p180-ErbB3
• p45-sErbB3
• p85-sErbB3
• Proto-oncogene-like protein c-ErbB-3
• receptor tyrosine-protein kinase erbB-3
• Tyrosine kinase-type cell surface receptor HER3
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERBB3