EPLIN Antibody (1B7)

Western Blot: EPLIN Antibody (1B7) [H00051474-M06] - Western Blot detection against Immunogen (91.89 KDa) .

Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06] - Analysis of monoclonal antibody to LIMA1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

Sandwich ELISA: EPLIN Antibody (1B7) [H00051474-M06] - Detection limit for recombinant GST tagged LIMA1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P, S-ELISA
• Clone: 1B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EPLIN Antibody (1B7) Summary

• Immunogen: LIMA1 (AAH01247, 1 a.a. ~ 598 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE
• Specificity: Reacts with LIM domain and actin binding 1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIMA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is reactive against recombinant protein in Western Blot and ELISA. Also works in IHC-Paraffin.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EPLIN Antibody (1B7)

• 1110021C24Rik
• Epithelial protein lost in neoplasm
• EPLINepithelial protein lost in neoplasm beta
• FLJ38853
• LIM domain and actin binding 1
• MGC131726
• SREBP3LIM domain and actin-binding protein 1
• sterol regulatory element binding protein 3

Background

EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIMA1
• Entrez:
  • 51474(Human)
• Uniprot:
  • AAH01247(Human)