EPB41L5 Antibody

Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NB100-93392] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: EPB41L5 Antibody [NB100-93392] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: EPB41L5 Antibody [NB100-93392] - (1ug/ml) staining of A431 (A) and (0.3ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NB100-93392] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EPB41L5 Antibody Summary

• Immunogen: Peptide with sequence C-ENLPQSPGTDQHD, from the internal region of the protein sequence according to NP_065960.2; NP_001171866.1; NP_001171868.1; NP_001317239.1.
• Epitope: C-ENLPQSPGTDQHD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPB41L5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 80kDa band observed in lysates of cell line A431 and approx. 75kDa in lysates of cell line MCF7 (calculated MW of 81.9kDa according to NP_065960.2). Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EPB41L5 Antibody

• band 4.1-like protein 5
• erythrocyte membrane protein band 4.1 like 5
• FLJ12957
• KIAA1548BE37
• YMO1

Background

EPB41L5 may contribute to the correct positioning of tight junctions during the establishment of polarity inepithelial cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPB41L5
• Entrez:
  • 57669(Human)
• Uniprot:
  • NP_065960.2(Human)