Epac2 Overexpression Lysate

Western Blot: Epac2 Overexpression Lysate (Adult Normal) [NBL1-15153] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Epac2.

• Reactivity: Hu
• Applications: WB

Epac2 Overexpression Lysate Summary

• Description:

  Epac2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC205335
  
  Accession#: NM_007023
  
  Protein Tag: C-MYC/DDK
• Gene: RAPGEF4

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 115.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Epac2 Overexpression Lysate

• cAMP-GEFII
• cAMP-regulated guanine nucleotide exchange factor II
• CGEF2
• CGEF2EPAC 2
• EPAC 2
• Epac2
• Exchange factor directly activated by cAMP 2
• Exchange protein directly activated by cAMP 2
• Nbla00496
• putative protein product of Nbla00496
• Rap guanine nucleotide exchange factor (GEF) 4
• rap guanine nucleotide exchange factor 4
• RAP guanine-nucleotide-exchange factor (GEF) 4
• RAPGEF4

Background

Epac2, also known as Rap Guanine Nucleotide Exchange Factor (GEF) 4, is a guanine nucleotide exchange factor for RAP1A, RAP1B and RAP2A small GTPases. Studies have shown that EPAC2 has a significant role in the development of type 2 diabetes. EPAC2 has also been studied in relation to ataxia, neuronitis, osteoporosis and pancreatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RAPGEF4