Enteropeptidase/Enterokinase Antibody (190034)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 190034
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Enteropeptidase/Enterokinase Antibody (190034) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Enteropeptidase/Enterokinase
  Leu41-His1019
  Accession # NP_002763
• Specificity: Detects human Enteropeptidase/Enterokinase in direct ELISAs and Western blots. It recognizes all EK forms containing the heavy chain including the single chain precursor and the mature heterodimer consisting of a heavy and light chain. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Factor X and rhThrombin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMPRSS15
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Enteropeptidase/Enterokinase Antibody (190034)

• EC 3.4.21
• EC 3.4.21.9
• Enterokinase
• Enteropeptidase
• ENTK
• ENTKenterokinase
• MGC133046
• protease, serine, 7 (enterokinase)
• PRSS7
• PRSS7enteropeptidase
• Serine protease 7
• TMPRSS15
• Transmembrane protease serine 15
• transmembrane protease, serine 15

Background

Enterokinase (EK), also called enteropeptidase and TMPRSS15 (gene name), initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond-linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enteropeptidase/Enterokinase Antibody (MAB1585)(6)

Publications using MAB1585

• Nishida, K;Matsumura, S;Uchida, H;Abe, M;Sakimura, K;Badea, TC;Kobayashi, T; Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons PloS one 2023-09-21 [PMID: 37733805]
• Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF; IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy Cell reports 2023-07-31 [PMID: 37527036] (IHC, Mouse)
• Ma W, Wei X, Gu H et al. Intra-amniotic transplantation of brain-derived neurotrophic factor-modified mesenchymal stem cells treatment for rat fetuses with spina bifida aperta Stem cell research & therapy 8/13/2022 [PMID: 35964077]
  
  Details:
  Dilution used 1:25, Outbred Wistar rats
• Guimaraes-Souza EM, Perche O, Morgans CW et al. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Exp Eye Res 5/1/2016 [PMID: 26719241]
• Zelinka C, Scott M, Volkov L, Fischer A The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE, 2012-09-04;7(9):e44477. 9/4/2012 [PMID: 22973454]
• Vilen ST, Nyberg P, Hukkanen M, Sutinen M, Ylipalosaari M, Bjartell A, Paju A, Haaparanta V, Stenman UH, Sorsa T, Salo T Intracellular co-localization of trypsin-2 and matrix metalloprotease-9: possible proteolytic cascade of trypsin-2, MMP-9 and enterokinase in carcinoma. Exp. Cell Res., 2007-11-12;314(4):914-26. 2007-11-12 [PMID: 18062964] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TMPRSS15
• Uniprot:
  • NP_002763()