ENT1 Antibody - BSA Free

Western Blot: ENT1 Antibody [NBP2-41313] - Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: ENT1 Antibody [NBP2-41313] - Human ovary tissue with ENT1 antibody at 5 ug/mL.

Immunofluorescence: ENT1 Antibody [NBP2-41313] - Immunofluorescence of SLC29A1 in human ovary tissue with SLC29A1 antibody at 20 ug/mL.

Product Discontinued

ENT1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid peptide near the center of human SLC29A1. The immunogen is located within amino acids 240 - 290 of SLC29A1. Amino Acid Squence: ISKGEEPRAGKEESGVS
• Specificity: SLC29A1 antibody is human specific. SLC29A1 antibody is predicted to not cross-react with other SLC29 proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC29A1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat: (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ENT1 Antibody - BSA Free

• ENT1
• ENT1es-type
• equilibrative nucleoside transporter 1
• MGC1465
• MGC3778
• SLC29A1
• solute carrier family 29 (nucleoside transporters), member 1
• Solute carrier family 29 member 1
• solute carrier family 29, member 1

Background

ENT1 is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC29A1