ENPP-2/Autotaxin Overexpression Lysate (Native)

Images

 
Western Blot: ENPP2 Overexpression Lysate (Adult Normal) [NBL1-10275] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ENPP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ENPP-2/Autotaxin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205377 and based on accession number NM_001040092. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), transcript variant 2, mRNA.
Gene
ENPP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ENPP-2/Autotaxin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ENPP-2/Autotaxin Overexpression Lysate (Native)

  • ATX
  • ATXFLJ26803
  • ATX-X
  • Autotaxin
  • autotaxin-t
  • EC 3.1.4.39
  • ectonucleotide pyrophosphatase/phosphodiesterase 2
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 2
  • E-NPP 2
  • ENPP2
  • ENPP-2
  • Extracellular lysophospholipase D
  • Lysophosphatidic Acid
  • LysoPLD
  • NPP2
  • PD-IALPHA
  • PDNP2
  • PDNP2NPP2
  • phosphodiesterase I/nucleotide pyrophosphatase 2
  • plasma lysophospholipase D

Background

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region, compared to variant 1, resulting in a shorter protein (isoform 2, also known as beta), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37477
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NBP2-19809
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
2984-SE
Species: Hu
NBP2-27561
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
MAB5756
Species: Hu
Applications: Flow
NBP1-03363
Species: Hu, Rt, Ch
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-84903
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-38952
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF6270
Species: Hu
Applications: ICC
NLS1014
Species: Hu, Mu, Rt, Bt, Bv, Ca, Eq, Ha, Mk, Pm
Applications: IHC-P
H00008720-M07
Species: Hu
Applications: ELISA, ICC/IF
NBP2-14101
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-15343
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82710
Species: Hu
Applications: IHC, IHC-P
NBL1-10275
Species: Hu
Applications: WB

Publications for ENPP-2/Autotaxin Lysate (NBL1-10275) (0)

There are no publications for ENPP-2/Autotaxin Lysate (NBL1-10275).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ENPP-2/Autotaxin Lysate (NBL1-10275) (0)

There are no reviews for ENPP-2/Autotaxin Lysate (NBL1-10275). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ENPP-2/Autotaxin Lysate (NBL1-10275) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ENPP-2/Autotaxin Products

Bioinformatics Tool for ENPP-2/Autotaxin Lysate (NBL1-10275)

Discover related pathways, diseases and genes to ENPP-2/Autotaxin Lysate (NBL1-10275). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ENPP-2/Autotaxin Lysate (NBL1-10275)

Discover more about diseases related to ENPP-2/Autotaxin Lysate (NBL1-10275).
 

Pathways for ENPP-2/Autotaxin Lysate (NBL1-10275)

View related products by pathway.

PTMs for ENPP-2/Autotaxin Lysate (NBL1-10275)

Learn more about PTMs related to ENPP-2/Autotaxin Lysate (NBL1-10275).

Blogs on ENPP-2/Autotaxin

There are no specific blogs for ENPP-2/Autotaxin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ENPP-2/Autotaxin Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ENPP2
Entrez