ENPP-2/Autotaxin Overexpression Lysate

Western Blot: ENPP2 Overexpression Lysate (Adult Normal) [NBL1-10275] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ENPP2.

• Reactivity: Hu
• Applications: WB

ENPP-2/Autotaxin Overexpression Lysate Summary

• Description:

  ENPP-2/Autotaxin Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC205377
  
  Accession#: NM_001040092
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: ENPP2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ENPP-2/Autotaxin Overexpression Lysate

• ATX
• ATXFLJ26803
• ATX-X
• Autotaxin
• autotaxin-t
• EC 3.1.4.39
• ectonucleotide pyrophosphatase/phosphodiesterase 2
• ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• E-NPP 2
• ENPP2
• ENPP-2
• Extracellular lysophospholipase D
• Lysophosphatidic Acid
• LysoPLD
• NPP2
• PD-IALPHA
• PDNP2
• PDNP2NPP2
• phosphodiesterase I/nucleotide pyrophosphatase 2
• plasma lysophospholipase D

Background

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region, compared to variant 1, resulting in a shorter protein (isoform 2, also known as beta), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ENPP2