Endophilin A1/SH3GL2 Antibody

Western Blot: Endophilin A1/SH3GL2 Antibody [NBP2-26164] - Staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Endophilin A1/SH3GL2 Antibody [NBP2-26164] - Staining of Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Endophilin A1/SH3GL2 Antibody Summary

• Immunogen: Peptide with sequence C-SLEFPTGDSTQPN corresponding to internal region according to NP_003017.1.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SH3GL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 40.0 kDa band according to NP_003017.1). IHC-P: Human cerebral cortex shows staining of cytoplasm in some but not all neuronal cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Endophilin A1/SH3GL2 Antibody

• CNSA2
• CNSA2FLJ25015
• EEN-B1
• EEN-B1bA335L15.1 (SH3-domain GRB2-like 2)
• Endophilin A1
• Endophilin-1
• endophilin-A1
• FLJ20276
• SH3 domain protein 2A
• SH3 domain-containing GRB2-like protein 2
• SH3D2A
• SH3D2AEndophilin A1 BAR domain
• SH3-domain GRB2-like 2
• SH3GL2
• SH3P4
• SH3P4endophilin-1

Background

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endophilin A1/SH3GL2 Antibody (NBP2-26164)(1)

Publication using NBP2-26164

• Shang C, Guo Y, Fu S et al. SH3GL2 gene participates in MEK-ERK signal pathway partly by regulating EGFR in the laryngeal carcinoma cell line Hep2. Med Sci Monit 2010-06-01 [PMID: 20512084]

Bioinformatics

• Gene Symbol: SH3GL2
• Entrez:
  • 6456(Human)