Endophilin A1/SH3GL2 Antibody

Western Blot: Endophilin A1/SH3GL2 Antibody [NBP2-20345] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL2 antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Endophilin A1/SH3GL2 Antibody [NBP2-20345] - SH3GL2 antibody [N1C1] detects SH3GL2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SH3GL2 stained by SH3GL2 antibody [N1C1] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Endophilin A1/SH3GL2 Antibody [NBP2-20345] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.

Product Discontinued

Endophilin A1/SH3GL2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SH3GL2. The exact sequence is proprietary.
• Localization: Cytoplasm; Membrane; Peripheral membrane protein
• Predicted Species: Human (100%), Chicken (93%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3GL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (87%), Rabbit (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Endophilin A1/SH3GL2 Antibody

• CNSA2
• CNSA2FLJ25015
• EEN-B1
• EEN-B1bA335L15.1 (SH3-domain GRB2-like 2)
• Endophilin A1
• Endophilin-1
• endophilin-A1
• FLJ20276
• SH3 domain protein 2A
• SH3 domain-containing GRB2-like protein 2
• SH3D2A
• SH3D2AEndophilin A1 BAR domain
• SH3-domain GRB2-like 2
• SH3GL2
• SH3P4
• SH3P4endophilin-1

Background

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SH3GL2
• Uniprot:
  • Q99962()