EN-RAGE/S100A12 Antibody (S100A12/8952R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human spleen stained with EN-RAGE/S100A12 Antibody (S100A12/8952R) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: S100A12/8952R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EN-RAGE/S100A12 Antibody (S100A12/8952R) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-95) of human S100A12 protein (exact sequence is proprietary) (Uniprot: P80511)
• Localization: Secreted
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S100A12
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for EN-RAGE/S100A12 Antibody (S100A12/8952R) - Azide and BSA Free

• CAAF1
• CAAF1Neutrophil S100 protein
• CAAFI
• CAGC
• CAGCS100 calcium binding protein A12 (calgranulin C)
• CAGCS100
• Calcium-binding protein in amniotic fluid 1
• Calgranulin C
• calgranulin-C
• CGRPEN-RAGE
• ENRAGE
• EN-RAGE
• Extracellular newly identified RAGE-binding protein
• MRP6
• p6calgranulin C
• protein S100-A12
• S100 calcium binding protein A12
• S100 calcium-binding protein A12 (calgranulin C)
• S100 calcium-binding protein A12
• S100A12

Background

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B--a key transcription factor for inflammatory events--is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A12