ELMO1 Antibody (JG34-59)

Western Blot: ELMO1 Antibody (JG34-59) [NBP2-76832] - Analysis of ELMO1 on mouse spleen tissue and Jurkat cell lysates using anti-ELMO1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: ELMO1 Antibody (JG34-59) [NBP2-76832] - Analysis of mouse spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow Cytometry: ELMO1 Antibody (JG34-59) [NBP2-76832] - Analysis of SH-SY-5Y cells with ELMO1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: ELMO1 Antibody (JG34-59) [NBP2-76832] - Analysis of rat spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Product Discontinued

ELMO1 Antibody (JG34-59) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ELMO1 aa 678-727 / 727. (SwissProt: Q92556 Human; SwissProt: Q8BPU7 Mouse; SwissProt: D3ZY46 Rat)
• Localization: Cell membrane, Cytoplasm, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ELMO1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ELMO1 Antibody (JG34-59)

• ced-12 homolog 1
• CED12CED-12
• ELMO-1
• engulfment and cell motility 1 (ced-12 homolog, C. elegans)
• engulfment and cell motility 1
• engulfment and cell motility protein 1
• KIAA0281MGC126406
• Protein ced-12 homolog

Background

ELMO1 is encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELMO1