ELA3A Overexpression Lysate (Native)

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Western Blot: ELA3A Overexpression Lysate (Adult Normal) [NBL1-10220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ELA3A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ELA3A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203249 and based on accession number NM_005747. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens elastase 3A, pancreatic (ELA3A), mRNA.
Gene
CELA3A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ELA3A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELA3A Overexpression Lysate (Native)

  • chymotrypsin-like elastase family member 3A
  • chymotrypsin-like elastase family, member 3A
  • EC 3.4.21
  • EC 3.4.21.70
  • ELA3A
  • ELA3elastase 1
  • elastase 3A, pancreatic (protease E)
  • elastase 3A, pancreatic
  • Elastase IIIA
  • elastase-3A
  • Protease E

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CELA3A