EIF5 Antibody

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Western Blot: EIF5 Antibody [NB100-93312] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used for WB at 0.04 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
   

Biological Strategies

     

Order Details

EIF5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 5 using the numbering given in entry NP_892116.2 (GeneID 1983).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is useful for western blot. It is not recommended for immunoprecipitation.
Positive Control
EIF5 Lysate (NBL1-10213)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF5 Antibody

  • eIF-5
  • EIF-5A
  • eukaryotic translation initiation factor 5

Background

Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF5
Entrez
Uniprot