EIF5 Antibody

Western Blot: EIF5 Antibody [NB100-93312] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used for WB at 0.04 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EIF5 Antibody Summary

• Description: Novus Biologicals Rabbit EIF5 Antibody (NB100-93312) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 5 using the numbering given in entry NP_892116.2 (GeneID 1983).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for western blot. It is not recommended for immunoprecipitation.
• Control:

  EIF5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF5 Antibody

• eIF-5
• EIF-5A
• eukaryotic translation initiation factor 5

Background

Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5
• Entrez:
  • 1983(Human)
  • 217869(Mouse)
• Uniprot:
  • NP_892116.2(Human)