Novus Biologicals products are now on bio-techne.com

eIF2B epsilon Overexpression Lysate

Images

 
Western Blot: eIF2B epsilon Overexpression Lysate (Adult Normal) [NBL1-10181] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF2B epsilon.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

eIF2B epsilon Overexpression Lysate Summary

Description

eIF2B epsilon Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202322

Accession#: NM_003907

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EIF2B5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
80.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for eIF2B epsilon Overexpression Lysate

  • CACH
  • CLE
  • eIF-2B GDP-GTP exchange factor subunit epsilon
  • EIF-2B
  • EIF2BE
  • EIF2Bepsilon
  • eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD)
  • eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
  • LVWM
  • translation initiation factor eIF-2B subunit epsilon

Background

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84877
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00008894-M09
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-16292
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-24813
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-02669
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF6260
Species: Hu
Applications: WB
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-560
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
NBP2-21577
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
H00056731-M02
Species: Hu
Applications: ELISA, ICC/IF, WB
NB100-81898
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NB200-157
Species: Hu
Applications: IHC, IHC-P, KO, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-83210
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB500-526
Species: Hu
Applications: ELISA, IHC, IHC-P, WB
NBP3-03965
Species: Hu, Mu, Rt
Applications: ICC/IF, WB

Publications for eIF2B epsilon Lysate (NBL1-10181) (0)

There are no publications for eIF2B epsilon Lysate (NBL1-10181).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eIF2B epsilon Lysate (NBL1-10181) (0)

There are no reviews for eIF2B epsilon Lysate (NBL1-10181). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eIF2B epsilon Lysate (NBL1-10181) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional eIF2B epsilon Products

Research Areas for eIF2B epsilon Lysate (NBL1-10181)

Find related products by research area.

Blogs on eIF2B epsilon

There are no specific blogs for eIF2B epsilon, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eIF2B epsilon Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF2B5