eIF2A [p Ser49] Antibody

Product Discontinued

eIF2A [p Ser49] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF2a around the phosphorylation site of Serine 49 (L-L-Sp-E-L).
• Modification: p Ser49
• Specificity: eIF2A (phospho-Ser49) detects endogenous levels of eIF2a only when phosphorylated at Serine 49.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100~1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500~1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF2A [p Ser49] Antibody

• EIF-2
• EIF-2A
• EIF2AEIF2
• EIF-2alpha
• eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• subunit 1 (alpha, 35kD )

Background

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2A
• Entrez:
  • 83939(Human)
  • 229317(Mouse)
  • 502531(Rat)
• Uniprot:
  • P05198()