EI24 Overexpression Lysate (Native)

Western Blot: EI24 Overexpression Lysate (Adult Normal) [NBL1-10166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EI24.

• Reactivity: Hu
• Applications: WB

EI24 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC201737 and based on accession number NM_004879. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens etoposide induced 2.4 mRNA (EI24), transcript variant 1, mRNA.
• Gene: EI24

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for EI24

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EI24 Overexpression Lysate (Native)

• EPG4
• etoposide induced 2.4 mRNA
• p53-induced gene 8 protein
• PIG8etoposide-induced protein 2.4 homolog
• TP53I8
• tumor protein p53 inducible protein 8

Background

This gene has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The protein encoded by this gene contains six putative transmembrane domains and may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in two transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: EI24