Eg5 Antibody

Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NBP2-76927] - ICC staining Eg5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Eg5 Antibody [NBP2-76927] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Eg5 antibody. Counter stained with hematoxylin.

Flow Cytometry: Eg5 Antibody [NBP2-76927] - Flow cytometric analysis of A431 cells with Eg5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NBP2-76927] - ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NBP2-76927] - ICC staining Eg5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Eg5 Antibody [NBP2-76927] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Eg5 antibody. Counter stained with hematoxylin.

Product Discontinued

Eg5 Antibody Summary

• Immunogen: Recombinant protein within Human Eg5 aa 80-257 / 1,056. (SwissProt: P52732 Human; SwissProt: Q6P9P6 Mouse)
• Localization: Cytoplasm, Cytoskeleton.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Eg5 Antibody

• Eg5
• HKSP
• KIF11
• kinesin family member 11
• kinesin-like 1
• Kinesin-like protein 1
• kinesin-like protein KIF11
• Kinesin-like spindle protein HKSP
• Kinesin-related motor protein Eg5
• KNSL1
• KNSL1TRIP-5
• thyroid receptor interacting protein 5
• Thyroid receptor-interacting protein 5
• TR-interacting protein 5
• TRIP5
• TRIP-5
• TRIP5EG5

Background

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF11