EFTUD2 Antibody (1-D10-A1)

Western Blot: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis on different cell lysates using anti-EFTUD2 Mouse mAb. Positive control: Lane 1: Jurkat Lane 2: NIH/3T3

Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Staining of SW480 cells using anti-EFTUD2 Mouse mAb.

Immunohistochemistry-Paraffin: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis of paraffin- embedded mouse prostate tissue using anti-EFTUD2 Mouse mAb.

Flow Cytometry: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis of MCF-7 cells with EFTUD2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ncubation with primary antibody; black). Goat anti mouse IgG (FITC) was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Staining of SH-SY-5Y cells using anti-EFTUD2 Mouse mAb

Immunohistochemistry-Paraffin: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis of paraffin- embedded human colon cancer tissue using anti-EFTUD2 Mouse mAb.

Immunohistochemistry-Paraffin: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis of paraffin- embedded human lung cancer tissue using anti-EFTUD2 Mouse mAb.

Immunohistochemistry-Paraffin: EFTUD2 Antibody (1-D10-A1) [NBP2-66818] - Analysis of paraffin- embedded mouse colon tissue using anti-EFTUD2 Mouse mAb.

Product Discontinued

EFTUD2 Antibody (1-D10-A1) Summary

• Immunogen: Synthetic peptide within mouse EFTUD2 aa 1-50 / 972. (SwissProt: O08810 Mouse)
• Localization: Nucleus
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFTUD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for EFTUD2 Antibody (1-D10-A1)

• DKFZp686E24196
• elongation factor Tu GTP binding domain containing 2
• Elongation factor Tu GTP-binding domain-containing protein 2
• FLJ44695
• hSNU114
• KIAA0031U5-116 kDa
• SNRP116
• Snrp116,116 kDa U5 small nuclear ribonucleoprotein component
• SNU114 homolog
• Snu114
• U5 snRNP specific protein, 116 kD
• U5 snRNP-specific protein, 116 kD
• U5 snRNP-specific protein, 116 kDa
• U5-116KD

Background

EFTUD2 is one of several proteins that make up the U5 small nuclear ribonucleoprotein (snRNP) particle, which is a component of the spliceosome. It contains consensus GTPase motifs, and it has been shown that this protein binds GTP specifically.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EFTUD2