eEF-2 Antibody (5B6)

Western Blot: eEF-2 Antibody (5B6) [NBP2-37620] - Western blot analysis using EEF2 mouse mAb against HepG2 (1), Hela (2), HEK293 (3) and A431 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: eEF-2 Antibody (5B6) [NBP2-37620] - Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: eEF-2 Antibody (5B6) [NBP2-37620] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.

Immunohistochemistry: eEF-2 Antibody (5B6) [NBP2-37620] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.

ELISA: eEF-2 Antibody (5B6) [NBP2-37620] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

eEF-2 Antibody (5B6) Summary

• Immunogen: Purified recombinant fragment of human EEF2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EEF2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for eEF-2 Antibody (5B6)

• eEF2
• eEF-2
• EF-2
• EF2EEF-2
• elongation factor 2
• eukaryotic translation elongation factor 2
• polypeptidyl-tRNA translocase

Background

The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF2