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ECM29 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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ECM29 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-KIAA0368 full-length
Gene
KIAA0368

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for ECM29

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-KIAA0368 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ECM29 Overexpression Lysate (Denatured)

  • ECM29 homolog, proteasome accessory protein
  • ECM29
  • FLJ22036
  • homolog of yeast Ecm29
  • KIAA0368
  • KIAA1962
  • proteasome-associated protein ECM29 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Perilipin-2/ADFP at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunocytochemistry/Immunofluorescence: VPS11 Antibody [NBP2-47583] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VPS11 Antibody [NBP2-47583] - Staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
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ECM29 Overexpression Lysate (Denatured)
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Applications: WB

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Bioinformatics

Gene Symbol KIAA0368